| 학회 | 한국화학공학회 |
| 학술대회 | 2002년 봄 (04/26 ~ 04/27, 강원대학교) |
| 권호 | 8권 1호, p.1225 |
| 발표분야 | 생물화공 |
| 제목 | Burkholderia multivorans DH4를 이용한 lipase 생산 |
| 초록 | Lipase is the enzyme which hydrolyzes the esters of long chain aliphatic acids from glycerol at oil/water interfaces. Recently lipases were studied intensively for the production of fatty acids and the interesterification of lipids. In order to find microorganisms which produce lipase, screening of microorganisms from waste water and sludge of oil industry was done. Newly isolated Burkholderia multivorans DH4 was cultured for the production of lipase. Optimal temperature, pH and agitation speed in the batch culture for the production of lipase were established. The optimal temperature, pH and agitation speed were 30℃, pH 6, 200rpm respectively. Medium for production of lipase in the batch culture was optimized. The optimal inoculum size was 4%. The optimized medium was determined to be 2% soluble starch, 1% casamino acid, 0.02% MgSO4·7H2O, 0.5% K2HPO4 and 2% olive oil. When Burkholderia multivorans DH4 was cultured in 250 ml shake flask with the optimized medium, 3.09 U/ml of lipase activity was obtained. A batch culture of Burkholderia multivorans DH4 was performed in 2.5L stirred tank reactor with the optimized medium. After 32 hr of cultivation the highest lipase activity (2.54 U/ml) was obtained. |
| 저자 | 이동환1, 김영권2, 김승욱 |
| 소속 | 1고려대, 2고려바이오 (연) |
| 키워드 | lipase; hydrolysis; optimization of medium |
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| 원문파일 | 초록 보기 |
