| 초록 |
Polymers can be used in diverse ways for tissue-engineering, including as a scaffold to provide appropriate mechanical and adhesive cues for tissue formation, or as a means to deliver bioactive molecules that control stem cell fate. We have used combinations of norbornene-functionalised gelatin (Gel-NB) and hyaluronic acid (HA-NB) together with polyethylene glycol (PEG) dithiol (PEG-(SH)2), to create both bulk hydrogels or microgels laden with mesenchymal stromal cells (MSCs) and shown effects of both hydrogel composition and geometry on bone differentiation. We have further examined the potential for polyethylenimine (PEI) nanoparticles to deliver pro-osteogenic miRNAs to cells. By incorporating these into a hydrogel, we demonstrate improved delivery of miRNAs compared to conventional transfection protocols, and show that this approach can be used as a simple one-pot system to enhance the osteogenesis of MSCs in hydrogels for bone tissue engineering. |
