| 초록 |
In biotechnology, electrospun nanofibers are utilized for biosensors, tissue regeneration scaffolds, and drug delivery systems. Among these fabricated nanofibrous scaffolds, poly(lactide-co-glycolide) (PLGA) fiber sheets have been used as scaffolds for tissue engineering. This is because of this material’s biodegradability, biocompatibility, and mechanical properties. However, PLGA fiber sheets alone are not enough for effective tissue regeneration. The gold nanoparticle (GNPs) have been widely used in many fields such as drug delivery, bio-sensing, and tissue-engineering due to their unique properties. GNPs also have a powerful bonding force with thiol-groups which allows them to be conjugated to a wide variety of materials, and have a high osteoinduction effect. For this reason, we designed and prepared a thiolated PLGA (PLGA-SH) nanofibrous sheet for binding with GNPs using a simple process. The objective this study was to design a manufacturing process for PLGA-GNPs nanofibrous sheet. This sheet can find many applications within the field of tissue engineering, more specifically orthopedic materials. The manufactured membrane was characterized by thermogravimetric analysis (TGA), Field emission scanning electron microscopy (FE-SEM), energy dispersive X-ray spectroscopy (EDX), X-ray photoelectron spectroscopy (XPS), Attenuated total reflectance fourier transform infrared spectroscopy (ATR-FTIR), and ultraviolet/visible spectroscopy (UV-vis). It was also evaluated for cytotoxicity and osteogenic effects using human adipose derived stem cells (hASC). Characterization results show that the GNPs are well attached on the PLGA-SH sheet and it is possible to control the amount of attached GNPs. In addition, the in vitro results showed that PLGA-GNPs had good biocompatibility and osteogenic effects towards human adipose derived stem cells. Through these results, we found that the PLGA-GNPs can be useful materials for bone regeneration and have additional usage as a controlled release carrier for drugs attached to the GNPs. |
