| 학회 |
한국화학공학회 |
| 학술대회 |
2005년 봄 (04/22 ~ 04/23, 여수대학교) |
| 권호 |
11권 1호, p.396 |
| 발표분야 |
생물화공 |
| 제목 |
Display of Lipase on the Cell Surface of Escherichia coli using OprF |
| 초록 |
We have developed a new cell surface display system using a major outer membrane protein of Pseudomonas aeruginosa OprF as an anchoring motif. P. fluorescens SIK W1 lipase gene was fused to the truncated oprF gene by C-terminal deletion fusion strategy. The truncated OprF-lipase fusion protein was successfully displayed on the surface of Escherichia coli. Localization of the truncated OprF-lipase fusion protein was confirmed by western blot analysis, immunofluorescence microscopy and whole cell lipase activity. To examine the enzymatic characteristics of the cell surface displayed lipase, whole cell enzyme activity and stability were determined under various conditions. Cell surface displayed lipase showed the highest activity at 37oC and pH 8.0. It retained over 80% of initial activity after incubation for a week in both aqueous solution and organic solvent. These results suggest that E. coli cells displaying lipases using OprF as an anchoring motif can be employed for various biotechnological applications both in aqueous and non-aqueous phases. (This work was supported by MOCIE grants from the Intelligence Bioinformatics and Application Center (TGW10011093) at the KRIBB) |
| 저자 |
최종현, 이승환, 이상엽
|
| 소속 |
한국과학기술원 생명화학공학과 및 생물공정연구센터 |
| 키워드 |
cell surface display; Escherichia coli; proteins; oprF
|
| E-Mail |
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| 원문파일 |
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