| 학회 |
한국화학공학회 |
| 학술대회 |
2003년 봄 (04/25 ~ 04/26, 순천대학교) |
| 권호 |
9권 1호, p.471 |
| 발표분야 |
생물화공 |
| 제목 |
Poly(3-hydroxybutyrate) Production by High Cell Density Culture of Ralstonia eutropha: Oxygen Supply and Consumption |
| 초록 |
A comparative fed-batch culture study of poly(3-hydroxybutyrate) (PHB) production by Ralstonia eutropha (R. eutropha) was carried out with air and high purity oxygen in 5-L, 30L, and 300-L fermentors. In the 30-L fermentor, the final dry cell weight and PHB concentrations obtained with high purity oxygen were 185.9 and 138.7g/L, which increased 3.8 and 6.2 times respectively as compared with the results obtained in the case of air supply. Whereas, the dry cell weight and PHB concentration in the 5-L fermentor increased 2.2 and 2.9 times respectively. They were mainly attributed to the enough oxygen supply in the case of high purity oxygen. It is impossible to meet the oxygen demand of cells in a large-scale high cell density culture by supplying air only. It was found that there was just about 4~5% oxygen consumed in the case of air supply, but it increased to 9~11% in the case of high purity oxygen supply. |
| 저자 |
Longan Shang, 임성춘, 김도윤, 장호남
|
| 소속 |
한국과학기술원 생명화학공학과 |
| 키워드 |
High cell density culture; Oxygen supply; Oxygen consumption; Poly(3-hydroxybutyrate)
|
| E-Mail |
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| 원문파일 |
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