| 학회 |
한국화학공학회 |
| 학술대회 |
2010년 가을 (10/21 ~ 10/22, 대전컨벤션센터) |
| 권호 |
16권 2호, p.1592 |
| 발표분야 |
생물화공 |
| 제목 |
Immobilization of His-tagged dual ring-fission oxygenases on nickel (Ⅱ) ion functionalized silica-magnetic nanoparticles for complete degradation of phenol |
| 초록 |
In some bacteria, biodegradation of phenol occurs with ring fission processes by mono- and di-oxygenases. This study shows an indirect method of protein immobilization by using Ni(Ⅱ) ion for specific interaction of metal-protein to conduct stable and renewable biocatalysts for complete phenol degradation. For this, silica-coated Fe3O4 magnetic nanoparticles(Si-MNPs) were functionalized with Ni(Ⅱ) ion(Ni/Si-MNPs) to immobilize histidine-tagged dual ring-fission oxygenases which are phenol-monooxygenase(PMO)and catechol 1,2-dioxygenase(CatA) originated from Corynebacterium glutamicum. After immobilization on the Ni/Si-MNPs, the apparent Km for the PMO and CatA were increased than free enzymes. The recyclability of immobilized enzymes was ~70% after recycling it up to six times. This approach also enabled us to develop the sequential removal process of phenol via catechol degradation indicating the applications in aquatic environments. |
| 저자 |
이수연1, 이진형1, 이선영2, 민지호3, 장정호1
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| 소속 |
1한국세라믹기술원 바이오-IT 융합센터, 2한국세라믹기술원 바이오-IT 융합센터 / 인하대, 3전북대 |
| 키워드 |
His-tagged dual ring-fission oxygenases; silica-magnetic nanoparticle; phenol degradation
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| E-Mail |
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| 원문파일 |
초록 보기 |
