| 초록 |
In this study, Carbonic anhydrase(CA) and Phosphoenolpyruvate Carboxylase(PEPCase) were immobilized and stabilized in magnetically-separable enzyme microbeads(EMBs) using electrospinning method. These two enzymes are crucial for CO2 mitigation and counteraction of greenhouse effect, because they simultaneously capture CO2 and utilize four-carbon compounds. This microbead composed of branched polymer(polyethylene glycol), magnetic bead, and silica shell for mechanical, long-term stability and recyclable property. By developing this integrated system, we successfully converted CO2 to oxaloacetate, which is organic acid and base compound for precursor of bio-polyester. This CA-PEPCase co-immobilized EMB retained high activity and stability. Furthermore, the magnet-based separation was also successful for the reuse of the EMBs. |
