| 학회 |
한국화학공학회 |
| 학술대회 |
2021년 가을 (10/27 ~ 10/29, 광주 김대중컨벤션센터) |
| 권호 |
27권 2호, p.1731 |
| 발표분야 |
생물화공 |
| 제목 |
Enhancement of malic acid production by introduction of protein colocalization strategy in recombinant Escherichia coli |
| 초록 |
To produce malic acid from non-oxidative pathway route in Escherichia coli using two key enzymes and synthetic scaffold complex. E. coli was engineered to produce malic acid from glucose by co-localization of two key enzymes phosphoenolpyruvate carboxylase (Ppc) and malate dehydrogenase (MdhA) with synthetic scaffold complex. Scaffold plasmid has produced the maximum concentration of 3.51 g/L malic acid from 10 g/L glucose in 48 h of culture. pH 5.5 and temperature 30°C were optimum for malic acid production without any engineering of competing metabolic pathways. E. coli mutant strains and different concentrations of glucose also tested. When 50 g/L glucose was used as substrate, 20.4 g/L of malic acid was produced. |
| 저자 |
Kumaravel Ashokkumar1, 정재훈2, 홍순호2
|
| 소속 |
1Univ. of Ulsan, 2울산대 |
| 키워드 |
생물화학공학 |
| E-Mail |
|
| 원문파일 |
초록 보기 |
