| 초록 |
The diarylethene derivative has been used in the reversible quenching of the fluorescence of a fluorophore through photochromic fluorescence resonance energy transfer. Here, we developed a biocompatible reversible photoswitching system based on the dendritic nanocluster, which was readily internalized into living cells and living zebrafish. These nanoclusters were prepared by oligomerizing dendrimers using a diarylethene derivative (FRET acceptor) as a crosslinker, followed by the attachment of a fluorophore (FRET donor). Interestingly, unlike the solution samples prepared in cuvettes (24-47%), a high degree of fluorescence quenching was achieved when dendritic nanoclusters were internalized into living systems (83-95%). We envision that our relatively nontoxic dendritic nanoclusters may facilitate fluorescence imaging in vivo by enhancing its resolution through the reversibly controlled exhibition of high on-off contrast. |
