| 초록 |
With the development of simple and rapid point-of-care testing (POCT) diagnostic platform, various types of microfluidic-based chips have been designed intensively. If plastics which can be mass-produced at low cost applied to these applications, the cost of the disease diagnosis will be much reduced, thus consequently bring massive economic benefits to patients. However, the surface of plastics is known to be hydrophobic, making it difficult to use on platform for bioassays. Hence, we aim to adopt a nature-inspired surface engineering using polydopamine to modify plastic surface, which enables incorporation of a silica coating to extract nucleic acids, specifically RNA. As a demonstration, we applied surface-modified plastic chips for enrichment and in situ amplification of RNA to determine antibiotic-resistance of bacteria. |
