| 초록 |
Biological molecules represent organisms’ physiological states and serve as biomarkers of diseases. Numerous methods have been reported to quantify them. Recently, research efforts have been made to develop point-of-care tests, which contribute to early diagnoses of diseases, particularly in resource-limited settings. Here, simple homogeneous methods based on proximity proteolysis reactions (PPR) are reported to detect biological molecules. A typical PPR system has been designed such that the proteolysis reaction between protease and zymogen is enhanced in the presence of a target analyte. The activated zymogen generates a color signal by hydrolyzing a chromophore. A protease and zymogen are linked to target binders using specific hybridization between complementary single-stranded DNAs, and several molecules, antibodies and aptamers, are used as target binders to detect target proteins. |
