| 초록 |
Protein adsorption onto the surface of a foreign material is the initial event that occurs when a surface comes into contact with the living fluid such as blood. In the protein adsorption, the intrinsic properties of surface and protein are essential factors and these properties could affect the molecular interaction between protein and surface. Despite the importance of molecular interaction between the protein and the surface, the strength of the molecular interaction is not well understood, mainly due to the technical difficulty involved in direct measurement of the strength of molecular interaction. The molecular force measurement using an atomic force microscope (AFM) provides a useful strategy for investigating the protein-surface interaction. Here, we measured the molecular force of representative blood plasma protein (bovine serum albumin (BSA)) to foreign material surfaces. Force-versus-displacement curves were measured using atomic force microscope (AFM) between the protein covalently immobilized AFM tip and the various surfaces. Firstly, we investigated the surface energy dependency on the molecular force of the protein. Secondly, the charge and hydrophobic effect on adhesion force was studied by changing the pH of the media. Finally, the temperature dependency on molecular force of the proteins to poly(N-isopropylacryamide) brush is investigated. |
