| 학회 |
한국재료학회 |
| 학술대회 |
2013년 봄 (05/23 ~ 05/24, 여수 엠블호텔(THE MVL)) |
| 권호 |
19권 1호 |
| 발표분야 |
D. 바이오재료(Biomaterials) |
| 제목 |
Chemically-Conjugated Bone Morphogenetic Protein-2 on porous PCL/BCP-Collagen scaffolds stimulates Osteogenic activity |
| 초록 |
The conjugation of recombinant human bone morphologenetic protein-2(rh-BMP-2) on absorbable porous PCL/BCP-Collagen scaffold was developed in this study. Immunofluorescent staining against BMP-2 demonstrated that BMP-2 was successfully immobilized on the PCL/BCP-Collagen three-dimensional scaffold by aminolysis and subsequent chemical conjugation. The ability of this system to deliver rhBMP-2 was tested for the repair of bone defect. The morphology and pore size distribution of PCL/BCP-Collagen and PCL/BCP-Collagen-BMP-2 was observed by Scanning Electron Microscope (SEM) and Mercury porosimetry. The in vitro evaluation of with and without rh-BMP-2 scaffolds was done by MTT assay, Live/dead cell and immunofluorescent with mouse calvaria-derived preosteoblastic cells (MC3T3-E1). The surface modification of PCL/BCP-Col scaffolds with BMP-2 showed the good ability to support the attachment and proliferation of the pre-osteoblast cells. The PCL-BCP-Collagen-BMP-2 could be used for cell and growth factor carriers for bone regeneration. |
| 저자 |
Ji Hyun Kim1, Nguyen Thuy Ba Linh2, Young Ki Min3, Hun Mo Yang1, Jun sang Bae2, Byong Taek Lee3
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| 소속 |
1Department of Biomedical Engineering & Materials, 2College of Medicine, 3Soonchunhyang Univ. |
| 키워드 |
PCL; BCP; BMP-2; Porous scaffold; aminolysis
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| E-Mail |
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