| 학회 |
한국화학공학회 |
| 학술대회 |
2019년 봄 (04/24 ~ 04/26, 제주국제컨벤션센터) |
| 권호 |
25권 1호, p.679 |
| 발표분야 |
이동현상(Transport Phenomena) |
| 제목 |
Multiplexed Protein Detection with Post-Synthesis Hydrogel Microparticles |
| 초록 |
Protein detection and quantification is increasingly becoming an important analytical practice in various applications from biopharmacy to medical diagnosis. With a growing demand for simultaneous measurement of multiple analytes in a single sample, suspension assay platforms have emerged at the forefront of multiplex protein detection. Hydrogel beads have been proposed with the purpose of integrating hydrogel’s solution-like reactivity and non-fouling characteristic. However, their synthesis is deemed unreliable and delicate because the photoinitiators used for radical polymerization of hydrogels are incompatible with probe antibodies and cause antibodies to aggregate. In this work, we introduce hydrogel microparticles that are functionalized after synthesis and demonstrate their advanced multiplex protein detection performance. Our detection technique features complete elimination of antibody aggregation, high sensitivity, fast detection, and broad assay range. |
| 저자 |
이낙준1, 이현지1, 노윤호1, 김현웅1, 김선민2, 봉기완1
|
| 소속 |
1고려대, 2보라매병원 |
| 키워드 |
이동현상 |
| E-Mail |
|
| 원문파일 |
초록 보기 |
