| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 가을 (10/30 ~ 11/01, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
23권 2호 |
| 발표분야 |
포스터_생체재료 |
| 제목 |
Polydiacetylene Coating Immobilized Ligands and Surface-Enhanced Raman Scattering-Embedded Beads for Detection without Label |
| 초록 |
Multiple detection technologies using protein are powerful tools to investigate the potein-protein interaction. Due to the efficiency and convenience, we used embedded beads to perform the experiments. However, effective detection of embedded bead is challenging because it is difficult to bind labeled secondary protein and target protein. In our previous research, polydiacetylene coated surface-enhanced Raman scattering-embedded beads(PDA–SERS beads) were invented and produced an enhanced encoding ability due to their SERS properties and label-free detection from the PDA layer. In this research, we introduced biotin conjugated PDA–SERS beads for detection target protein model (streptavidin) in the PDA-SERS bead system. Biotin-conjugated PDA–SERS beads showed high fluorescence intensity by the increasing concentration of streptavidin. This method allows detecting streptavidin with detection limit of 2ⅹ10-8 M throughout the fluorescence intensity without labelling procedure. |
| 저자 |
성보미, 함은일, 조아라, 안재현, 김윤희, 전봉현
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| 소속 |
건국대 |
| 키워드 |
Surface Enhanced Raman Scattering; Polydiacetylene (PDA); Label-Free Detection
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| E-Mail |
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