| 학회 |
한국화학공학회 |
| 학술대회 |
2012년 봄 (04/25 ~ 04/27, 제주 ICC) |
| 권호 |
18권 1호, p.419 |
| 발표분야 |
생물화공 |
| 제목 |
Immobilization-free SELEX assisted by Graphene Oxide |
| 초록 |
Aptamers are ssDNA or RNA oligonucleotides with high affinity and high specificity to their targets. We have used graphene oxide (GO) to obtain aptamers which bind to model protein, Nampt. The π-π interaction of ssDNA to GO forms the basis for simplified, immobilization-free approach to systematic evolution of ligands by exponential enrichment (SELEX). The GO in the GO-SELEX is utilized as a material tool that can not only separate unbound ssDNA from the target protein-aptamer complex in the SELEX process but also recover the target inducing released ssDNA from the GO surface in the counter-SELEX process by affinity based desorption. Thus relatively cheap single layer GO sheets can be used and applied to identify aptamers specific to any target of interest. In this presentation, new aptamer screening method assisted by GO and the affinity and specificity of these finally screened aptamers will be discussed. |
| 저자 |
박지웅1, Rameshwar Tatavarty1, 김대우2, 정희태2, 구만복1
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| 소속 |
1고려대, 2KAIST |
| 키워드 |
aptamer; SELEX; Graphene oxide
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| E-Mail |
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| VOD |
VOD 보기 |
| 원문파일 |
초록 보기 |
