| 초록 |
We developed fluorescent OFF-ON-OFF probe using Cu2+ functionalized fluorescent carbon dots (FCD) to obtain (Cu2+–FCD) for a selective targeting of membrane and nucleus in cancer cell. The interaction of FCD and Cu2+ resulted in aggregation, leading to fluorescence quenching. In tumor cells, Cu2+–FCD was disintegrated by emulative binding with stronger affinity to intracellular PPi for Cu2+, enhancing fluorescent signal. After that, the PPi–Cu2+ complex was hydrolyzed by intracellular alkaline phosphatase in the cytoplasm to release Cu2+, causing re-aggregation with FCD that observed by fluorescence re-quenching behavior. Therefore, the system has shown the potential as a sensitive and selective tumor diagnostic platform in both coated and in aqueous states. |
