| 초록 |
In the present, high cost in the petroleum market, green house effect, and the fossil fuel reliability are great problems, and Bio-ethanol is the best solution to these problems. Ethanol has been trusted as an alternate fuel for the future. The ethanologenic pathway in Z. mobilis (Zymomonas mobilis), like that of saccharomyces cerevisiae, consist of two essentail activities, pyruvate decarboxylase(pdc) and alcohol dehydrogenase (ADH). These two activities and the enzymes of glycolysis comprise 30 to 50% of the soluble protein in Z. mobilis. By inserting Z. mobilis genes encoding pyruvate decarboxylase and alcohol dehydrogenase II in E. coli. E. coli was able to ferment sugars into ethanol. This study Z. mobilis (containing pdc and adhB genes) were used as the construction sources of genes and plasmids. Expression vectors carrying pdc and adhB genes were constructed by using pET-32a vectors. Ethanol productivity in E. coli strain seemed to be affected by the extent of expresstion of pdc gene along with adhB genes. By successful gene mutation we established a new E. coli strain which can produce ethanol efficiently by fermentation in aerobic conditions. |
