| 학회 |
한국고분자학회 |
| 학술대회 |
2008년 봄 (04/10 ~ 04/11, 컨벤션 뷰로(대전)) |
| 권호 |
33권 1호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
Bio-imaging of LYVE-1 Receptor-Mediated Endocytosis of Hyaluronic Acid Derivatives Using Quantum Dots |
| 초록 |
Hyaluronic acid (HA) plays important roles in the body through the interaction with receptors on cells. Among the receptors, LYmphatic Vessel Endothelial hyaluronan recepor-1 (LYVE-1) abundantly exist in the liver and the lymphatics with a high specificity to HA. We investigated the effect of chemical modification of HA on its LYVE-1 receptor-mediated endocytosis using quantum dots (QDots). B16F1 cells expressing LYVE-1 were incubated with HA-QDot and analyzed with a confocal microscope and a well plate reader. The results clearly showed a reduction of internalization via receptor-mediated endocytosis with the increasing degree of HA modification. Using near-infrared emission QDots, we confirmed that HA-QDot conjugates with a low degree of HA modification maintaining enough binding sites for HA receptors were mainly accumulated in the liver, while those with a high degree of HA modification losing much of HA characteristics were evenly distributed to the body. |
| 저자 |
김기수, 김지석, 강현구, 한세광
|
| 소속 |
포항공과대 |
| 키워드 |
Bio-imaging; LYVE-1; Endocytosis; Hyaluronic Acid; Quantum Dots
|
| E-Mail |
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