| 초록 |
Delayed re-endothelialization and impaired endothelial function are link to the thrombosis and restenosis of cardiovascular implant. Therefore, the rapid re-endothelialization is the main focus in the increasing strategies for cardiovascular applications. Herein, polyvinyl chloride (PVC) substrate was immobilized with heparin and copper nanoparticles (CuNPs), a NO-catalytic agent, to enhance the attachment and functionalization of endothelial cells. Heparin-tyramine (HT) and CuNPs were immobilized onto PVC substrate via tyrosinase-mediated reaction. The amount of immobilized heparin and released NO were controllable by varying the feeding concentration of HT and CuNPs. In vitro studies revealed that the HT/Cu coatings sustainedly released NO (0.2-18 µM for 14 days) resulting in the decrease of platelets and smooth muscle cells adhesion, and the increase of endothelialization. In conclusion, HT/Cu coating is expected to be useful in supporting the treatment of cardiovascular diseases. |
