| 학회 |
한국공업화학회 |
| 학술대회 |
2022년 봄 (05/11 ~ 05/13, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
26권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Reconstruction of galactose utilization pathway for cadaverine production in E. coli |
| 초록 |
Biological production of cadaverine has been suggested as an alternative route to replace polyamides generated by the petroleum-based process. As most microorganisms cannot efficiently metabolize other biomass-derived sugars as fast as glucose, redesigning microorganisms is necessary for utilizing those carbon sources with enhanced carbon flux and product formation. In this study, we engineered E. coli to produce cadaverine with rapid assimilation of galactose, a promising future feedstock. To achieve this, genes related to the metabolic pathway were maximally expressed to amplify the flux toward cadaverine. The feedback inhibition of metabolic enzymes and degradation/re-uptake pathways was also inactivated to robustly produce cadaverine. The final strain DHK4 produced 8.80 g/L cadaverine with high yield and productivity during fed-batch fermentation, which was similar to or better than the previous glucose fermentation. This is the first report to produce cadaverine from galactose. |
| 저자 |
백동엽1, 곽동훈1, 임현규1, 양진아2, 서상우3, 정규열1
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| 소속 |
1POSTECH, 2제주대, 3서울대 |
| 키워드 |
galactose; red macroalgae; cadaverine; biorefinery; Escherichia coli
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| E-Mail |
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