| 학회 |
한국공업화학회 |
| 학술대회 |
2022년 봄 (05/11 ~ 05/13, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
26권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Structural study on the impact of S239D/I332E mutations in the binding of Fc and FcγRIIIa |
| 초록 |
Engineering of Fc for improved affinity to its receptor, can enhance the therapeutic activity of monoclonal antibodies. S239D/I332E mutation of Fc has been employed in various Fc engineering studies, but is not clear how the mutations have structurally influenced the molecular interactions between Fc-FcγRIIIa. In this study, the point or combined mutations of S239D/I332E were introduced into one chain (A) or the other chain (chain B) of the homodimeric Fc domain computationally. Their structural effects on the binding to FcγRIIIa were investigated through docking methods. These results showed that the chain-specific point mutation, S239D induced a new salt-bridge with the receptor in A and B chains of Fc, whereas I332E mutation generated a new salt-bridge with the receptor only in A chain. The combined mutation study reproduced the three salt-bridges.This study is expected to provide more structural insight into Fc variants’ design based on S239D/I332E mutation. |
| 저자 |
Jebamani Petrina, Sun-Gu Lee
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| 소속 |
부산대 |
| 키워드 |
Fc; FcγRIIIa; in silico mutation; protein-protein docking; salt-bridges
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| E-Mail |
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