| 초록 |
Influenza virus entry is promoted by multivalent interaction of influenza hemagglutinin with sialic acid, which is a main residue of host cell receptor. Therefore, conjugation of sialyl ligands to nano-scaffolds is one of the effective strategies to develop inhibitors to block influenza virus cell entry. This led us to investigation of optimal spacing between sialyl groups in the multivalent ligand for effective viral inhibition. In this study, we developed a range of synthetic sialyl ligands with controlled-valencies and spacing. Sialyl groups were coupled to lysine-based dendritic molecules via various lengths of flexible linkers, which were confirmed by 1H-NMR and mass spectroscopy. Next, antiviral activities of the sialyl ligands were investigated by hemagglutination inhibition assay and fluorescence-based neuraminidase inhibition assay. Based on these results, we investigated relationship between sialyl ligand structure and inhibition efficiency. |
