| 학회 |
한국화학공학회 |
| 학술대회 |
2015년 봄 (04/22 ~ 04/24, 제주 ICC) |
| 권호 |
21권 1호, p.371 |
| 발표분야 |
생물화공 |
| 제목 |
Cofactor-free Light-driven Whole-cell Cytochrome P450 Catalysis |
| 초록 |
Cytochrome P450s can catalyze various regioselective and stereospecific oxidation reactions of non-functionalized hydrocarbons. Here, we have designed a novel light-driven platform for cofactor-free, whole-cell P450 photo-biocatalysis using eosin Y (EY) as a photosensitizer. EY can easily enter into the cytoplasm of Escherichia coli and bind specifically to the heme domain of P450. The catalytic turnover of P450 was mediated through the direct transfer of photo-induced electrons from the photosensitized EY to the P450 heme domain under visible light illumination. A proof-of-concept for the photoactivation of the P450 catalytic cycle in the absence of cofactors and redox partners is successfully conducted using bacterial P450s (variants of P450 BM3) and human P450s (CYPs 1A1, 1A2, 1B1, 2A6, 2E1, and 3A4) for the bioconversion of various substrates, including marketed drugs (simvastatin, lovastatin, and omeprazole) and a steroid (17β-estradiol), to demonstrate the general applicability of the light-driven, cofactor-free system. |
| 저자 |
박종현1, 이상하1, 차건수2, 최다솜1, 남동헌1, 이재형1, 이정걸3, 윤철호2, 박찬범1, 정기준1
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| 소속 |
1KAIST, 2전남대, 3건국대 |
| 키워드 |
Cytochrome P450; Eosin Y; Photosensitizer; Escherichia coli
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| E-Mail |
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| VOD |
VOD 보기 |
| 원문파일 |
초록 보기 |
