| 초록 |
Highly selective, sensitive, and multiple detection sensor have become a critical challenge for disease diagnosis. In this article, we describe a multi-analyte biosensor for the detection of cancer biomarkers based on plasmon response of single gold nanoparticles (AuNPs) and antibody-antigen binding interaction. To demonstrate the ability to detect and quantify cancer biomarkers: prostate speacific antigen (PSA), -fetoprotein (AFP), Carcinoembryonic antigen (CEA), a novel and sensitive multiplex sensor was developed by site-selectively immobilizing plasmonic nanoparticles functionalized with an each monoclonal antibodies that recognize the target protein on hydrophilic-hydrophobic patterned glass slide. The proposed multi-analyte biosensor exhibited outstanding selectivity and sensitivity. Using this sensing strategy, we find that multi-analyte ability, high sensitivity, simple, and ease of use of the detection method based on Rayleigh scattering spectrum of individual gold nanoparticles make it an excellent application for versatile molecular diagnostics in both research and clinical medical fields. |
