| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 가을 (10/13 ~ 10/14, 제주 ICC) |
| 권호 | 30권 2호 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Release of Gentamicine Sulfate and Silver-sulfadiazine in the Small Intestinal Submucosa |
| 초록 | Small intestine submucosa (SIS) is the natural biomaterials mainly composed of collagen, glycosaminoglycan and growth factors. Tissue engineering techniques have been investigated the possibility of the application of SIS for a wound dressing. It has been recently recognized that SIS has been examined as organ reconstructions without immuno-rejection response. In this study, the SIS sponge was prepared by crosslinked with 1-ethyl-(3-3-dimethyl amino propyl) carbodiimide hydrochloride (EDC) solution with 50 and 100 mM concentration for 24 hrs and lyophilized. Also, we loaded gentamicine sulfate and silver-sulfadiazine in SIS sponge using the micropipet after gentamicine sulfate and silver-sulfadiazine solution. For the examination of release pattern, SIS sponge was dipped in phosphate buffered saline (pH 7.4) and released at shaking incubator (37oC). At predetermined time intervals, each sample was analyzed using HPLC. In conclusion, 1% SIS sponge (1-100) showed faster release pattern than 2% SIS sponge (2-100). Furthermore, the release profile of the SIS sponge cresslinked with 50 mM EDC (1-50 and 2-50) showed fast initial burst in contrast with the sponge crosslinked with 100 mM EDC (1-100 and 2-100). As a result of this study, the SIS sponge containing drug released within 48 hours, and we can controll the release pattern of drug by regulating the amount of SIS and EDC. Acknowledgement : This work was supported by KMOHW (0405-BO01-0204-036). Figure 1. Release of Gentamicine Sulfate loaded SIS sponge. Reference 1. M. F. Cote, G Laroche, E. Gagnon, P. Chevallier, and C. J. Doillon, Biomaterials, 25, 3761 (2004). 2. T. Nakamura, Y. Inada, S. Fukuda, M. Yoshitani, A. Nakada, S. Itoi, S. Kanemaru, K. Endo and Y. Shimizu, Brain Res., 1027, 18 (2004). |
| 저자 | 송인범1, 홍금덕1, 이민숙1, 김문석2, 조선행2, 강길선1, 이해방2 |
| 소속 | 1전북대, 2한국화학(연) |
| 키워드 | Gentamicine Sulfate; Silver-sulfadiazine; SIS; release pattern |
