| 초록 |
Nanofibrous nonwoven matrices were prepared by electrospinning the regenerated silk fibroin (SF) solution, and structural changes of water vapor-treated SF nanofibers were investigated in a time-resolved manner using a custom-made infrared spectroscopy. Conformational transitions of SF nanofibers occurred in 1 hr following water vapor treatment at room temperature. SEM pictures indicated no remarkable morphological change occurred during water vapor treatment. However, water vapor treatment provided stronger and less brittle nanofiber matrices formation, compared with aqueous methanol-treated nanofiber matrices. The physical properties of the nanofiber matrices were greatly altered by treatment with water vapor that induced changes in the crystalline structure of the fibroin nanofibers, which was confirmed by time-resolved FT-IR and solid-state CP-MAS 13C NMR spectroscopy. This approach to monitoring the conformational changes of SF nanofibers in a real-time scale may may find useful applications in biomedical engineering areas, including would dressings and tissue regeneration. |
