| 학회 |
한국고분자학회 |
| 학술대회 |
2017년 봄 (04/05 ~ 04/07, 대전컨벤션센터) |
| 권호 |
42권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 I |
| 제목 |
Photothermal mediated gene delivery using IR780 loaded bovine serum albumin coated cell penetrating peptide conjugated disulfide crosslinked polyethylenimine polyplex in breast cancer cells. |
| 초록 |
Photo-triggered gene release can play a pivotal role in minimizing these limitations and achieve high gene transfection efficiency with minimum toxicity. In the current study, a photothermally controlled gene releasing nanocomplex was developed by complexing peptide conjugated ssPEI polyplex with IR780 loaded bovine serum albumin (BISPD). This BISPD nanocomplex forms a stable nano-sized complex with plasmid DNA and shows higher gene transfection efficiency without any sign of cell toxicity in 4T1 and 4T7 breast cancer cells. Moreover, the BISPD nanocomplex demonstrates an enhanced gene transfection efficiency upon NIR irradiation from 808 nm laser, which is attributed to accelerated endosomal escape of polyplexes augmented by locally induced heat in endosomal vesicles. To investigate the endosomal escaping effect of the BISPD nanocomplex, Bafilomycin A1 was employed and it confirmed the endosomal escape was mediated by the local heat generated by BISPD complex upon irradiation. |
| 저자 |
Rajendrakumar Santhosh Kalash1, Cherukula Kondareddy1, Saji uthaman2, 박인규1
|
| 소속 |
1Chonnam National Univ. Medical School, 2Chungnam National Univ. |
| 키워드 |
Photothermal; gene delivery; Bovine serum albumin; IR780; cancer
|
| E-Mail |
|
