| 초록 |
In recently study, non-viral vector have a attention in gene therapy. For enhanced gene delivery, it need effective transfection function and biocompatible ability. We investigated stabilized plasmid-lipid particle(SPLP) as a enhanced gene delivery vector. SPLP is liposomal encapsulation which have several advantage to overcome barrier of gene delivery. Generally, SPLP have a relatively low transfection than other vectors. We induced lysine residue for cell-permeable in cellular and expected increased transfection efficiency. SPLP is composed of various lipids. First, PEG-lipid was a material that consists of (PEG5000-Lys7-(Palmitic acid)2. And DOPE was formed for stabilized particle. The cationic lipid DOTAP was important characteristic to encapsulate plasmid DNA. We were prepared vesicle using Detergent dialysis method and were confirmed particle formation by diameter size. SPLP were purified by DEAE chromatography. Cell transfection was analyze using luciferase assay in HEK293 cell. |
