| 초록 |
Sugars are particularly interesting group of polyfunctional compounds. They are biologically relevant and contain multiple hydroxyl groups which are nearly indistinguishable chemically, yet differentiable enzymatically. Their combination into polymers is a natural event, leading to a various field of polysaccharides. Sugars have also been modified enzymatically and incorporated into polymers quite distinct from polysaccharides. Thereby, the esterification of sugars may have considerable impact in the synthesis of food intergredients, surfactants, sweeteners, and biochemical and pharmaceutical intermediates. Sugar esters, which are produced by esterification of sugar donor with acyl donor, are biocompatible, biodegradable, non-ionic and non-toxic, and can be synthesized from renewable sources. The specific properties of sugar ester depend on the acyl donor, the sugar moiety, the degree of substitution, and its chemical structure. In particular, sugar esters have been reported in the field of biomaterial and optical material [1, 2].For the past few years, several researchers have investigated the lipase-catalyzed synthesis of sugar-containing acrylic esters due to their biomedical applicability [3, 4]. Acrylic acid has a high hydrophilic characteristic due to its carboxylic group capable of esterifying with the hydroxyl group of sugars. Moreover, it has a vinyl group that can be polymerized. Vinyl acrylate has also been shown to act as irreversible acylation agent [5], and to be contained property of acrylic acid. Therefore, acrylic esters are expected to be efficient monomers for sugar polymers having high hydrophilicity and biocompatibility.In this work, the enzymatic esterifications of acrylic acid/vinyl methacrylate with b-methylglucoside were carried out by Novozym 435, lipase from Candida antarctica, as a biocatalyst. Optimal reaction conditions for the esterificatin of b-methylglucoside and acrylic acid/vinyl methacrylate such as reaction media, enzyme amount, molar ratio of substrates, initial substrate concentration and reaction temperature were determined. |
