| 학회 |
한국화학공학회 |
| 학술대회 |
2011년 가을 (10/26 ~ 10/28, 송도컨벤시아) |
| 권호 |
17권 2호, p.1488 |
| 발표분야 |
생물화공 |
| 제목 |
Heparin-coated superparamagnetic nanoparticle-mediated adeno-associated virus delivery for decreasing the time of cellular transduction |
| 초록 |
Gene delivery vectors based on adeno-associated virus (AAV) are highly promising due to several desirable features of this virus thatissuitablefor the delivery of foreign genes into both dividing and non-dividing cells. This study describes a new method involving adsorption of AAV to heparin-coated superparamagnetic nanoparticles (HpNP) to infection. AAV-HpNPswere employed to transduce cells on the magnetic field.Infectivity waschangedby Magnetic exposure time and AAV with varying ratios of HpNP. This system enables to transduce the cells by similargene expression level of the typical bolus method even in very short time, 1/8 of bolus. The magnetofection using AAV-HpNPs showed the transduction efficiency increase without compromising the cell viability. Neurite outgrowth in PC12 cells is significantly increased by AAV that encode NGF production-HpNP. AAV-HpNP using limited magnetic field area resulted in site-specific localization of transgene expression. AAV-HpNP suggested that the efficiency of therapeutic vector strategies is improved. |
| 저자 |
황준호, 장재형, 이슬기림, 김정숙, 김은미, 이창하, 안익성
|
| 소속 |
연세대 |
| 키워드 |
adeno-associated virus; superparamagnetic nanoparticle; magnetofection; gene delivery; viral vector
|
| E-Mail |
|
| 원문파일 |
초록 보기 |
