| 초록 |
Understanding the organ-wide molecular architectures of proteins is essential to dissecting the mechanisms of diseases, such as brain disorders and cancers, and for answering scientific questions. Recently, a process called ‘expansion microscopy’ was discovered, which increases the resolution of conventional microscopy four-fold by physically expanding specimens. In this process, a swellable hydrogel network is synthesized inside the specimens, and then expanded in deionized water followed by a specimen disruption process to homogenize the mechanical properties of the specimens. Here, we study the relationship between the degree of the sample disruption and the expansion factor of various organs. Through this study, we decide the optimum sample disruption process of each organ to expand various organs with a minimum distortion due to the inhomogeneous mechanical properties. |
