| 학회 |
한국화학공학회 |
| 학술대회 |
2017년 가을 (10/25 ~ 10/27, 대전컨벤션센터) |
| 권호 |
23권 2호, p.1739 |
| 발표분야 |
생물화공 |
| 제목 |
Directed evolution of the 3-hydroxypropionic acid production pathway by engineering aldehyde dehydrogenase using a synthetic selection device |
| 초록 |
3-Hydroxypropionic acid (3-HP) is an important platform chemical, and biological production of 3-HP from glycerol using glycerol dehydratase (GDHt) and aldehyde dehydrogenase (ALDH) has been revealed to be effective. Despite the successful attempts of 3-HP production, the biological process has problems arising from low activity and inactivation of the enzymes. To apply directed evolution to engineer the biosynthetic pathway, we constructed a synthetic selection device using a 3-HP-responsive transcription factor. It was applied to ALDH library, specifically aldehyde-binding site library of alpha-ketoglutaric semialdehyde dehydrogenase (KGSADH). After only two serial cultures, the enriched strains showing increased 3-HP production, and isolated KGSADH variant enzyme exhibited 2.79-fold higher catalytic efficiency toward its aldehyde substrate than the wild-type. Our approach provides the simple and efficient tool to engineer the pathway enzymes in metabolic engineering. |
| 저자 |
석주연1, 정규열2, 유태현3, 양진아4, 최상진3, 김경진5, 박성훈6
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| 소속 |
1포항공과대, 2포항공화대, 3아주대, 4서울대, 5경북대, 6UNIST |
| 키워드 |
생물화공 |
| E-Mail |
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| 원문파일 |
초록 보기 |
