| 학회 |
한국공업화학회 |
| 학술대회 |
2018년 가을 (10/31 ~ 11/02, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
22권 2호 |
| 발표분야 |
생체재료_포스터 |
| 제목 |
Preparation and Evaluation of Hyaluronic acid-coated ternary complex for Targeted Gene delivery |
| 초록 |
Branched polyethylenimine(bPEI) was a high gene transfection agent. However, bPEI utilization as gene carrier was limited because of high cytotoxicity. To overcome these obstacle, chitosan was introduced to bPEI by CDI as coupling agent. The chemical structure of chitosan-g-bPEI(CSPEI) was analyzed by 1H-NMR. The ternary complexes with pDNA, CSPEI, and hyaluronic acid (HA) was prepared by electrostatic charge interaction in aqueous phase. Also, its gel retardation assay showed that pDNA was completely bound by CSPEI and HA. In addition, pDNA from ternary complexes was protected against DNase. Beside, its transfection with CD44-receptor overexpressed cancer cell showed that ternary complexes with HA was increased more than binary complexes without HA. These results suggest that ternary complex is superb gene carrier with targeting effect against CD44-receptor overexpressed cancer cell line. (No. NRF-2016H1D5A1910499). |
| 저자 |
홍웅길1, 정경원1, 안준혁1, 박준규2, 정영일3, 나재운1
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| 소속 |
1순천대, 2(주)시지바이오, 3양산 부산대 |
| 키워드 |
Hyaluronic acid; Ternary complex; Targeted Gene delivery; Transfection
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| E-Mail |
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