| 학회 |
한국화학공학회 |
| 학술대회 |
2022년 봄 (04/20 ~ 04/23, 제주국제컨벤션센터) |
| 권호 |
28권 1호, p.1260 |
| 발표분야 |
[주제 12] 화학공학일반(부문위원회 발표) |
| 제목 |
Thermoresponsive fiber-based microwell scaffolds capable of formation and retrieval of salivary gland stem cell spheroids for the regeneration of damaged salivary glands |
| 초록 |
3D spheroid culture enhances cell-cell interactions and promotes the expression of stem cell properties, yet application of the spheroids is challenging. We fabricated a thermoresponsive fiber-based scaffold for formation and retrieval of spheroids with enhanced expression of stem cell-related genes than salivary gland stem cells in a monolayer culture(SGSCmonolayer). SGSC-derived spheroids(SGSCspheroid) were then implanted into the submandibular glands of mice after fractionated X-ray irradiation. The enhanced production of submandibular acini specific mucin and preservation of MIST1+ mature acinar cells were observed in SGSCspheroid group. SGSCspheroid group exhibited greater amelioration of tissue damage, preservation of KRT7+ terminally differentiated luminal ductal cells and less cellular damage than SGSCmonolayer group. A significant increase in Ki67+ proliferative acinar cells were noted only in SGSCspheroid group. Our results suggest that this scaffold facilitates the production, retrieval and delivery of SGSCspheroids to damaged salivary glands for therapeutic purpose. |
| 저자 |
안수정, 홍혜진, 조재민, 윤여준, 최도진, 이수현, 이화정, 김도현, 박재욱, 고원건, 임재열
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| 소속 |
연세대 |
| 키워드 |
생물화공(Biochemical Engineering) |
| E-Mail |
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| 원문파일 |
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