| 학회 |
한국화학공학회 |
| 학술대회 |
2018년 가을 (10/24 ~ 10/26, 대구 EXCO) |
| 권호 |
24권 2호, p.1471 |
| 발표분야 |
생물화공(Biochemical Engineering) |
| 제목 |
Metabolic Engineering of Escherichia coli for 3-Hydroxypropionic Acid and Malonic Acid production via β-Alanine Route |
| 초록 |
For the production of industrially valuable chemicals, 3-hydroxypropionic acid (3-HP) and malonic acid (MA) using β-alanine (BA) route, E. coli was metabolically engineered. First, various downstream enzymes were screened to produce malonic semialdehyde (MSA). The P. aeruginosa pa0132 (encoding BA pyruvate transaminase) was selected to generate MSA from BA. In order to produce 3-HP from MSA, E. coli ydfG gene (encoding MSA reductase) was introduced to the strain. The 3-HP titer was increased to 3.69 g/L in flask culture through additional overexpression of sdhC gene by replacing native promoter of sdhC gene with the trc promoter. For the production of MA, E. coli yneI was introduced into the strain. The MA titer was increased to 0.450 g/L in flask culture through additional deletion of the ydfG gene. Fed-batch culture of the final strain allowed production of 31.1 g/L 3-HP or 3.60 g/L MA from glucose. (This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Science and ICT through the National Research Foundation (NRF) of Korea (NRF-2012M1A2A2026556 and NRF-2012M1A2A2026557)). |
| 저자 |
이언희1, 송찬우2, 김제웅1, 조인진1, 이상엽1
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| 소속 |
1KAIST, 2GS Caltex |
| 키워드 |
생물화공 |
| E-Mail |
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| 원문파일 |
초록 보기 |
