| 학회 |
한국화학공학회 |
| 학술대회 |
2019년 봄 (04/24 ~ 04/26, 제주국제컨벤션센터) |
| 권호 |
25권 1호, p.398 |
| 발표분야 |
생물화공(Biochemical Engineering) |
| 제목 |
Production of 2,4-dihydroxybutyrate from glucose via homoserine |
| 초록 |
2,4-Dihydroxybutyrate(DHB) has diverse applications in the production of fine and specialty chemicals. This study aims to develop recombinant Escherichia coli W3110 for production of DHB from glucose via homoserine via two reactions; (i)conversion of homoserine to 2-oxo-4-hydroxybutiric acid(OHB), and (ii)reduction of OHB to 2,4-dihydroxybutyric acid [1]. To develop an efficient strain, screening and engineering of the enzymes for the two reactions were conducted. Seventeen transaminase and 10 dehydrogenases from various sources were tested and two best enzymes were selected. When E. coli W3110 was introduced by these two enzymes, production of DHB from glucose was confirmed. |
| 저자 |
Le Quoc Thai1, 박성훈2, Toan Vo Minh1, Nam Nguyen Hoai3
|
| 소속 |
1School of Energy and Chemical Engineering, 2UNIST, 3UNIST / School of Chemical and Biomolecular Engineering |
| 키워드 |
생물화학공학 |
| E-Mail |
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| 원문파일 |
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