| 초록 |
Lycopene is red colored carotenoid found in red fruits and vegetables. We developed a lycopene producing E. coli through MEP pathway. Firstly, we introduced isoprenoid synthesis enzyme such as Dxs, IspA, Idi and CrtEBI in wild type E. coli. One of the important features of lycopene is that it is accumulated to the cellular membrane because lycopene is highly hydrophobic. Therefore, high cell density culture can be helpful for production of lycopene. We found out that sugar (glucose) transporter mutant showed increased biomass yield and lowered acetate overflow in microorganism when glucose is used as sole carbon source. Therefore we constructed main glucose uptake pathway in E. coli. As the uptake rate of glucose decreases, maximum growth and yield is inhanced though growth rate was deteriorated. When the transcription level of several genes of parental strain and mutants were compared, TCA cycle, respiration and pentose phosphate pathway were up-regulated in sugar transporter mutants. |
