| 초록 |
Most bacteria have evolved to maximize growth rate, with rapid consumption of carbon sources. However, fast growth feature secretion of organic acid or ethanol and reduce biomass yield in aerobic conditions. Several glucose uptake pathways exist in Escherichia coli, and when these transporters or their subunits were knocked out, the growth and glucose uptake rates decreased but acetate overflow was relieved and biomass yield was improved. Alteration of metabolism caused by the mutations was investigated with transcriptome analysis and 13C metabolic flux analysis. As a result, various perturbations were identified in the sugar transporter mutants. Based on these findings, robust production hosts were constructed. When pathway genes for producing value-added compounds were introduced, the specific yields of the compounds were improved significantly in the sugar transporter mutants, suggesting that controlling the sugar uptake rate is a good strategy for improving metabolite production. |
