| 초록 |
During the past decade, two-photon microscopy (TPM) has emerged as a new, indispensable imaging tool to study of biology and medicine. TPM uses two near-infrared (NIR) photons as the excitation source and offers several advantages, including inherent sectioning capability, low photo-damage, longer observation time, and greater tissue penetration depth. Recently, we have developed ratiometric TP probes possessing a significant two-photon excited fluorescence, a marked emission color change, easy loading, high photostability, and low cytotoxicity. These probes can precisely detect thiols, H2S, ROS, pH, and enzyme activity6 in live cells as well as living tissues. The design and evaluation of a selection of our recent studies, and their use in medical research will be presented. |
