| 학회 |
한국공업화학회 |
| 학술대회 |
2016년 봄 (05/02 ~ 05/04, 여수 엑스포 컨벤션) |
| 권호 |
20권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Cell-free synthesis of functional cell-penetrating scFv in a cell-free protein synthesis system derived from Escherichia coli |
| 초록 |
In this study we attempted to express the scFv antibodies that had been engineered to penetrate into the cells and give signal through split GFP complementation. Cell-free protein synthesis system provides a high accessibility to the translational mechanism, thereby providing optimal environments to increase the productivity and solubility of scFv. To assist the formation of correct disulfide bonds, we have changed redox conditions of the cell extract and reaction mixture using GSSG/GSH redox buffer. To increase the solubility, we used a cell extract enriched with DsbA, DsbC, FkpA and SurA. We also used the leader sequence ubiquitin,in front of the target scFv and achieved a high level expression of functional scFv antibodies. The functionality has confirmed by reading the fluorescence signal of transfected cells in a black plate assay |
| 저자 |
박유진1, Christy Catherine1, Devi Kasi1, 이경호1, 신승민2, 김용성2, 김동명1
|
| 소속 |
1Chungnam National Univ., 2Ajou Univ. |
| 키워드 |
Cell-free protein synthesis system; scFv antibody; Escherichia coli
|
| E-Mail |
|
