| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 봄 (05/10 ~ 05/12, 광주 김대중컨벤센센터(Kimdaejung Convention Center)) |
| 권호 |
21권 1호 |
| 발표분야 |
생물공학 |
| 제목 |
Reassignment of AGG Sense Codon with Unnatural Amino Acids in Escherichia Coli |
| 초록 |
The biological protein synthesis system has been engineered to incorporate unnatural amino acid into proteins, and this has opened up new routes for engineering proteins with novel compositions. While such systems have been successfully applied in research, there remains a need to develop new approaches with respect to the wider application of unnatural amino acids. In this study, we reported a strategy for incorporating unnatural amino acids into proteins by reassigning one of the Arg sense codons, the AGG codon. Using this method, several unnatural amino acids were quantitatively incorporated into the AGG codon. Furthermore, we applied the method to multiple AGG sites, and even to tandem AGG sequences. The method developed and described here could be used for engineering proteins with diverse unnatural amino acids, particularly when employed in combination with other methods. |
| 저자 |
이병성1, 신승건1, 전종엽1, 장경순2, 이분열1, 최상돈1, 유태현1
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| 소속 |
1아주대, 2한국기초과학지원(연) |
| 키워드 |
Unnatural amino acid; Protein engineering; tRNA
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| E-Mail |
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