| 초록 |
To enhance intracellular uptake of gene-based vaccines and vaccine adjuvants, diverse formulation techniques have been investigated. In this study, we formulated sub-micron sized polymeric particles for improved intracellular uptake of gene-based vaccines and vaccine adjuvants via phagocytosis to antigen presenting cells. We designed polymeric hydrogels using thiolated linear polyethyleneimine and hyaluronic acids with a size around 500 nm. Cyclic dinucleotides were incorporated within hydrogels as immune-stimulating ligands. Cytokine release and biocompatibility was evaluated in macrophage cells after treatment of hydrogels into macrophage cells. In vivo, model antigen, ovalbumin, were injected with hydrogels, which showed elevated release of Type 1 interferon. Accordingly, hydrogels with cyclic dinucleotides can be harnessed as vaccine adjuvants due to their excellent induction of Type 1 interferon in mammalian cells. |
