| 초록 |
Highly accurate and prompt monitoring of stress hormone is imperative for preventing stress-related diseases. Simultaneously, developing a robust sensing platform is required for improving the detection ability with greatly enhanced accessibility and operability. To challenge this goal, in this study, we present a novel electrochemical biosensor for a cortisol detection harnessing an electrochemical platform comprising a reduced graphene oxide (rGO) electrode, a bifunctional protein interlayer, and an antibody probe specifically recognizing cortisol molecules. As a result, electrochemical impedance spectroscopic measurements show that the constructed sensing platform exhibits tens of pico-molar sensitivity for cortisol detection with a wide linear dynamic range spanning from 10 pM to 100 nM. In addition, employing the bifunctional d-BSA interlayer and antibody probe molecules enables to have a high selectivity against cortisol analogs, such as aldosterone and progesterone. |
