| 초록 |
α-gal epitopes are mainly responsible for hyperacute rejection(HAR) after pig-to-primate xenotransplantation since they are interacted with anti-Gal antibodies in human serum. To inhibit the immune response in human body through natural antibodies, the synthetic α-gal antigens are required. We synthesized three different type of α-gal - polystyrene(PS) bead complex as potential inhibitors of HAR. For the synthesis, both chemical and enzymatic methods are developed in this study. First, chemically modified mono or disaccharide was synthesized. And then, α-galactosyl oligosaccharides was sequentially synthesized by recombinant galactosyltransferases in engineered E.coli. Finally, the α-gal oligosaccharide was coupled with the polymer support which will be used for packing materials on-column. The glycosyltransferases used to synthesize oligosaccharides are a truncated bovine α-1,3-GalT (80-368), β1,4-GalT originated from Helicobacter pylori and β1,3-GlcNAcT originated from Neisseria gonorrhoeae. |
