| 학회 |
한국화학공학회 |
| 학술대회 |
2013년 가을 (10/23 ~ 10/25, 대구 EXCO) |
| 권호 |
19권 2호, p.1505 |
| 발표분야 |
생물화공 |
| 제목 |
Enhanced production of synthetic spider dragline silk protein in Escherichia coli |
| 초록 |
Naturally found spider silk, showing highly repeated structure and extreme size as well as strength, show attraction to many industrial applications. However, these characteristics hinder its expression in heterologous hosts by creation of extensive secondary structure from the repetitive sequences in mRNA, and the structures decrease ribosome processivity and facilitate mRNA degradation. We present strategies to solve biological problems that occur using the naturally found protein, spider dragline silk protein: increasing available ribosome pool and stabilizing mRNA to stop degradation. From the results, we were able to provide insight into approaches to control translation efficiency of proteins containing high molecular weight and highly repetitive sequence. [This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries (NRF-2012-C1AAA001-2012M1A2A2026556) and the Intelligent Synthetic Biology Center through the Global Frontier Project (2011-0031963) of the Ministry of Education, Science and Technology (MEST) through the National Research Foundation of Korea.] |
| 저자 |
정해나, 이상엽, Xiao-Xia Xia, 나도균
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| 소속 |
KAIST |
| 키워드 |
spider silk protein; metabolic engineering
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| E-Mail |
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| 원문파일 |
초록 보기 |
