| 초록 |
Among yeasts, Saccharomycees cerevisiae is the best-studied organism, and the most frequently employed yeast in industrial processes. 1,2-propandiol(1,2-PD) is used in unsaturated polyester resins, cosmetics, phar-maceuticals and as an environmentally friendly antifreeze and deicer. In this study Saccharomyces cerevisiae YPH500, E. coli MG1655 (containing mgs gene), and Citrobacter freundii (containing dhdD gene) were used as the construction sources of genes and plasmids. In the previous study we could develop new Saccharomyces cerrevisiae strains which can produce 1,2-PD. 1,2-PD productivity in Saccharomyces cerevisiae strain seemed to be increased by constructing co-expression vectors;; The pESC-URA vector containing two promoters, GAL1 and GAL10, which differs in the direction of transcription was used in the co-expression. Furthermore, the increase of 1,2-PD yield was induced, by adding input time and the amount of galactose which is controlled by the GAL promoter, to the fermentation process. |
