| 초록 |
Research on target protein delivery and skeletal tissue engineering will be presented in this seminar. In the study of protein delivery, poly(ε-caprolactone) (PCL) and gelatin were electrospun to fabricate tissue-engineered scaffolds with different fiber morphologies. These scaffolds were subsequently conjugated with heparin to immobilize a bioactive molecule by electrostatic interactions. The quantity of heparin conjugation was controlled by the crosslinking time and the fiber morphologies. The heparin-conjugated scaffolds might achieve higher loading efficiency and cumulative release of proteins than the non-conjugated ones over the course of a test. Especially, a growth factor strongly bound to the heparin-conjugated fibers was able to significantly induce proliferation of the smooth muscle cells, and the larger fibers functionalized with heparin and a growth factor would induce the greatest amount of cellular infiltration into the scaffold. Recently, the interfacial regions existing at skeletal tissues have been emphasized for the effective transmission of mechanical force and the reduction of stress concentration. In the study of ligament-to-bone (LTB) regeneration, LTB-relevant cells were isolated and inoculated in a porous cylindrical scaffold consisting of tendon and interface regions. The heparin-based hydrogel was employed to deliver cells and bone morphogenetic protein-2 (BMP-2) to promote regeneration of interface tissue. As a result, it was possible to create the unique stratification of calcified fibrocartilage and ligament tissues on a single scaffold in terms of biochemical and biomechanical characteristics. |
