| 초록 |
To develop polymeric drug delivery carriers for cancer therapy, arginine-grafted poly(amido ethyleneimine)/heparin complex was formulated. Poly(amido ethyleneimine) was polymerized by Michael addition of N,N-cystaminebisacrylamide (CBA) and N-Boc-1,6-diaminohexane (DAH). To increase cellular uptake, arginine was conjugated to the primary amine group of poly(CBA-DAH). AGP/heparin/vinblastine complex (30/1/0.6 weight ratio) has the proper particle size (less than 200 nm) for the EPR effect. The disulfide bonds of drug delivery carrier could be cleaved by intracellular reducing agents such as glutathione in the cytoplasm. It was measured the concentration of the released amount of drug by UV visible spectrophotometer at 262nm. As a result of the release test, the concentration of released vinblastine was gradually increased. To access the possibility of application for drug delivery system, cytotoxicity was tested in various cell line. Cytotoxicity was confirmed by MTT assay. |
