| 초록 |
Injectable hydrogels have been widely utilized as therapeutic implants and vehicles of drugs, proteins and cells for therapeutic applications, as they have several advantages based on minimally invasive injection procedure. In our group, various types of in situ forming hydrogels have been developed as injectable bioactive matrices, which have been applied for various tissue regeneration and protein/gene delivery. The hydrogel matrices were made of various synthetic and natural polymers, including PEO-PPO copolymers (e.g. Tetronic and Pluronic), gelatin, chitosan, hyaluronic acid, and heparin.These polymers were functionalized with phenol-derivatives, which can be crosslinked to form the hydrogels via enzyme-mediated reaction using horseradish peroxidase (free/immobilized HRP system) in the present of hydrogen peroxide (H2O2) under physiological conditions, and the physico-chemical properties of the gel matrices could be controlled by varying material parameters. In addition, we have also developed in situ forming nanogels (Heparin-Pluronic and Tetronic-derivatives) for drug and gene delivery. These In situ gelling systems have been extensively evaluated using various peptides, proteins, and cell types as well as in vivo animal models. Obtained results demonstrated that the injectable hydrogels/nanogels have a great potential for use as injectable materials for tissue regeneration and protein/gene delivery. |
